Tuberculosis (TB) has been a major research topic for our team over the past years. TB is an infectious disease and it's essential to incorporate techniques to diagnose TB and its drug susceptibility pattern as early as possible. The upsurge of TB has been accompanied by the rise in drug-resistant Mycobacterium tuberculosis (DR-MTB) strains. Currently, a collaborative study is being carried out with a neighbouring country, Pakistan, to study the prevalence of Multi-Drug Resistant Tuberculosis (MDR-TB) in both countries. We genotypically characterised the DR-TB strains to identify DR-TB-associated host biomarkers in Sri Lankan and Pakistani TB patients for the two most potent first-line drugs, Rifampin and Isoniazid.